Wound healing is a complicated process that is characterized by angiogenesis, granulation tissue formation, collagen deposition, epithelialization and wound contraction. All these phases involve complex biomolecular interactions among soluble cytokines, formed blood elements, the extracellular matrix and cells.
Ideally, repaired skin should be cosmetically and functionally identical to normal skin, which requires that the repaired wound not only be restored to normal anatomical structures, but also that it reaches an acceptable level functionally.
Over the years, a number of scientists have explored the better methods to promote wound healing.
Recently, antibacterial effect of drugs containing silver nanoparticles(AgNPs) are very attractive because the antibiotic resistance of a plenty of pathogenic bacteria and conditional pathogenic bacteria increases significantly.
Suppression and destruction of bacteria by silver can be performed by both mechanical membrane destruction by forming complex between surface atoms of silver particles and membranal components of bacteria, and electrical membrane destruction through the membrane potential change.
Recently, silver nanoparticles were prepared by using several nanotechnological methods, and it was demonstrated that these silver nanoparticles were more effective than silver ions(silver nitrate, silver sulphadiazine)
At low concentration, AgNPs not only are nontoxicity, broad-spectrum antibacterial, but also suppress and destroy various bacteria and viruses including HIV-1, promote wound healing.
It was demonstrated that AgNPs-treated skin was similar with normal one through macroscopical and histological tests.
Several effective AgNPs wound dressings have been developed and applied to the practical treatment all over the world.
AgNPs wound dressings are recognized as the most effective one among all the other treatment methods for the wound management.
A research team in the basic medicine faculty, Pyongyang Medical College,
The vapor transport volume of AgNPs wound dressing is 5.45±0.30㎎/(㎠∙h), more than transpiration volume of normal skin[1~2㎎/(㎠∙h)], but exhibits vapor outflow-suppressing effect comparing with fourfolds of gauze and open control respectively.[19.94±1.42, 56.89±5.45㎎/(㎠∙h)].
There isn't any significant differenc e in the vapor transport volume between the alginate dressing[5.50±0.29㎎/(㎠∙h)] and AgNPs wound dressing, showing that the vapor transport volume of AgNPs wound dressing is determined by alginate.
In order to prevent pool of wound secretion, wound dressing should have high water absorption rate. The water absorption rate of our AgNPs wound dressing is 252.2%, which is accessible to its use.
The size of bacteriostat zone increases according to the AgNPs concentration, and there are significant differences in 500, 1000㎍/㎖ of AgNPs concentration respectively.
AgNPs in the wound dressing release continuously so as to exhibit antibacterial effect and even the surface of dressing is covered by dead bacteria.
We found out the effect of various wound dressings(physical saline solution gauze, fibrin dressing, AgNPs wound dressing) on the wound healing in rat wound model.
Wound tissues are filled with granulation tissue at 7th, 8th, 9th days in AgNPs wound dressing, fibrin dressing, physical saline solution gauze respectively while not at even 9th days in untreated control.
The wound area decreases significantly from 4th day comparing with untreated control and becomes 0 at 10th day in AgNPs wound dressing, 6th day and 12th day respectively in fibrin one.
In physical saline solution gauze, it tends to decrease up to 18th day, but not significant comparing with untreated control.
While wound healing was completed in 10~12th day, 13~15th day in AgNPs wound dressing and fibrin dressing respectively, the convalescences were prolonged in physical saline solution gauze and untreated control.
By all accounts on several parameters, AgNPs wound dressing has the most powerful antibacterial effect, wound secretion absorption rate, wound healing promotion effect among all other dressings.
Our investigation on the clinical validation of AgNPs wound dressing continues.
